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hcaec nucleofector kit  (Lonza)


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    Structured Review

    Lonza hcaec nucleofector kit
    Hcaec Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcaec+nucleofector+kit/hcaec+nucleofector+kit/pm31955977-60-5-4
    Average 90 stars, based on 1 article reviews
    hcaec nucleofector kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Intragenic transcriptional interference regulates the human immune ligand MICA
    Article Snippet: Reporter assays were carried out by co‐transfection of firefly luciferase reporter plasmid carrying MICA promoter fragments and control pRL‐SV Renilla luciferase plasmid, followed by cell lysis and luciferase assay using the Dual‐Luciferase Reporter Assay System (Promega) and a TD‐2020 luminometer (Turner Designs). .. 293T, HeLa and HT1080 cells were transfected using GeneJuice and primary human arterial endothelial cells were transfected using HCAEC Nucleofector Kit (Lonza), and cells were lysed 48 h post‐transfection. ..

    Article Title: Intragenic transcriptional interference regulates the human immune ligand MICA.
    Article Snippet: Reporter assays were carried out by co-transfection of firefly luciferase reporter plasmid carrying MICA promoter fragments and control pRL-SV Renilla luciferase plasmid, followed by cell lysis and luciferase assay using the Dual-Luciferase Reporter Assay System (Promega) and a TD-2020 luminometer (Turner Designs). .. 293T, HeLa and HT1080 cells were transfected using GeneJuice and primary human arterial endothelial cells were transfected using HCAEC Nucleofector Kit (Lonza), and cells were lysed 48 h post-transfection. ..

    Article Title: Hyperglycemia-induced endothelial dysfunction is alleviated by thioredoxin mimetic peptides through the restoration of VEGFR-2-induced responses and improved cell survival.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Intragenic transcriptional interference regulates the human immune ligand MICA
    Article Snippet: .. Transient CRISPR Primary human arterial endothelial cells or fibroblasts were transfected with CRISPR plasmids targeting the MICA upstream promoter (pair 1: PX458‐C40/C42 or pair 2: PX458‐C41/C43), or HLA‐B exon1 (PX458‐C50/C52) or PDPN exon2 (PX458‐C14) as negative controls by nucleofection using the HCAEC Nucleofector Kit or NHDF Nucleofector Kit, respectively (Lonza). .. Cells were cultured for 3 days before being lifted for flow cytometric analysis of surface MICA expression, or for cell sorting of GFP‐positive cells directly into Trizol‐LS using a MoFlo MLS sorter (Beckman Coulter) for RNA analysis.

    Article Title: Intragenic transcriptional interference regulates the human immune ligand MICA.
    Article Snippet: .. Primary human arterial endothelial cells or fibroblasts were transfected with CRISPR plasmids targeting the MICA upstream promoter (pair 1: PX458-C40/C42 or pair 2: PX458-C41/C43), or HLA-B exon1 (PX458-C50/C52) or PDPN exon2 (PX458-C14) as negative controls by nucleofection using the HCAEC Nucleofector Kit or NHDF Nucleofector Kit, respectively (Lonza). .. Cells were cultured for 3 days before being lifted for flow cytometric analysis of surface MICA expression, or for cell sorting of GFP-positive cells directly into Trizol-LS using a MoFlo MLS sorter (Beckman Coulter) for RNA analysis.

    Article Title: Compositions and methods for regulating arterial tone
    Article Snippet: .. The vessel lumen was perfused with Krebs-HEPES buffer supplemented with 1% BSA to flush out red blood cells and 100 μL of Nucleofector transfection reagent (HCAEC Nucleofector kit, Lonza) containing 10 nM control, Hb α or CytB5R3 siRNAs (see Table below) was perfused into the lumen. ..

    Article Title: Intragenic transcriptional interference regulates the human immune ligand MICA
    Article Snippet: .. Primary human arterial endothelial cells or fibroblasts were transfected with CRISPR plasmids targeting the MICA upstream promoter (pair 1: PX458‐C40/C42 or pair 2: PX458‐C41/C43), or HLA‐B exon1 (PX458‐C50/C52) or PDPN exon2 (PX458‐C14) as negative controls by nucleofection using the HCAEC Nucleofector Kit or NHDF Nucleofector Kit, respectively (Lonza). .. Cells were cultured for 3 days before being lifted for flow cytometric analysis of surface MICA expression, or for cell sorting of GFP‐positive cells directly into Trizol‐LS using a MoFlo MLS sorter (Beckman Coulter) for RNA analysis.

    CRISPR:

    Article Title: Intragenic transcriptional interference regulates the human immune ligand MICA
    Article Snippet: .. Transient CRISPR Primary human arterial endothelial cells or fibroblasts were transfected with CRISPR plasmids targeting the MICA upstream promoter (pair 1: PX458‐C40/C42 or pair 2: PX458‐C41/C43), or HLA‐B exon1 (PX458‐C50/C52) or PDPN exon2 (PX458‐C14) as negative controls by nucleofection using the HCAEC Nucleofector Kit or NHDF Nucleofector Kit, respectively (Lonza). .. Cells were cultured for 3 days before being lifted for flow cytometric analysis of surface MICA expression, or for cell sorting of GFP‐positive cells directly into Trizol‐LS using a MoFlo MLS sorter (Beckman Coulter) for RNA analysis.

    Article Title: Intragenic transcriptional interference regulates the human immune ligand MICA.
    Article Snippet: .. Primary human arterial endothelial cells or fibroblasts were transfected with CRISPR plasmids targeting the MICA upstream promoter (pair 1: PX458-C40/C42 or pair 2: PX458-C41/C43), or HLA-B exon1 (PX458-C50/C52) or PDPN exon2 (PX458-C14) as negative controls by nucleofection using the HCAEC Nucleofector Kit or NHDF Nucleofector Kit, respectively (Lonza). .. Cells were cultured for 3 days before being lifted for flow cytometric analysis of surface MICA expression, or for cell sorting of GFP-positive cells directly into Trizol-LS using a MoFlo MLS sorter (Beckman Coulter) for RNA analysis.

    Article Title: Intragenic transcriptional interference regulates the human immune ligand MICA
    Article Snippet: .. Primary human arterial endothelial cells or fibroblasts were transfected with CRISPR plasmids targeting the MICA upstream promoter (pair 1: PX458‐C40/C42 or pair 2: PX458‐C41/C43), or HLA‐B exon1 (PX458‐C50/C52) or PDPN exon2 (PX458‐C14) as negative controls by nucleofection using the HCAEC Nucleofector Kit or NHDF Nucleofector Kit, respectively (Lonza). .. Cells were cultured for 3 days before being lifted for flow cytometric analysis of surface MICA expression, or for cell sorting of GFP‐positive cells directly into Trizol‐LS using a MoFlo MLS sorter (Beckman Coulter) for RNA analysis.

    Control:

    Article Title: Compositions and methods for regulating arterial tone
    Article Snippet: .. The vessel lumen was perfused with Krebs-HEPES buffer supplemented with 1% BSA to flush out red blood cells and 100 μL of Nucleofector transfection reagent (HCAEC Nucleofector kit, Lonza) containing 10 nM control, Hb α or CytB5R3 siRNAs (see Table below) was perfused into the lumen. ..



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